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Table 4 Differentially expressed proteins (DEPs) in the D. pulex clones G and M under starvation stress

From: The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements

Spot ID Gene ID Function R P R P SP Mre Mrp pIe pIp SC (%) NP
  24 h 48 h  
501 299795 3-Phosphoglycerate kinase 1.6 0.12 2.2 0.00 83.4 44.2 5.6 5.5 39.4 28
550 300012 Actin and related proteins 1.4 0.08 0.6 0.02 60.5 41.8 5.3 5.3 14.3 4
752 300012 Actin and related proteins 2.7 0.04 1.0 0.96 28.2 41.8 5.6 5.3 36.3 17
749 305550 Actin and related proteins 1.8 0.18 0.5 0.02 29.1 41.8 5.5 5.3 28.9 10
3309 306442 Actin and related proteins 1.1 0.30 1.8 0.01 62.6 41.9 5.4 5.2 30.8 8
417 100611 α-Tubulin 1.4 0.16 1.5 0.01 82.3 46.1 5.4 5.0 23.8 8
405 301837 α-Tubulin 1.0 0.95 1.5 0.03 81.9 49.9 5.3 5.0 33.0 16
416 301837 α-Tubulin 0.8 0.17 1.6 0.04 81.9 49.9 5.2 5.0 11.8 6
543 220693 Arginine kinase 1.7 0.09 2.6 0.00 57.6 39.9 5.7 5.5 58.4 32
757 220693 Arginine kinase 1.8 0.04 0.8 0.06 28.8 39.9 5.4 5.5 36.3 9
463 210624 Calreticulin 0.6 0.05 0.7 0.06 16 72.4 46.6 4.7 4.5 48.3 18
742 303899 Carboxypeptidase A2 0.6 0.03 0.9 0.31 17 30.0 46.1 4.8 5.2 29.6 14
908 300446 Cytosolic fatty-acid binding protein 1.0 0.93 0.3 0.03 26.1 14.8 6.0 5.3 31.1 4
805 231271 FKBP-type peptidyl-prolyl cis-trans isomerase 0.9 0.81 0.6 0.03 17 23.3 23.4 4.5 4.5 66.7 14
794 317266 Glutathione transferase 1.7 0.02 1.2 0.03 25.0 25 5.4 5.2 67.3 18
779 309746 H+-transporting two-sector ATPase 1.8 0.04 1.8 0.07 26.5 56.8 5.9 5.5 64.2 20
722 248155 Peptidase S1, chymotrypsin 2.5 0.04 1.7 0.02 15 39.9 30.2 5.5 4.7 22.3 3
845 9558 Ubiquitin and ubiquitin-like proteins 0.7 0.04 0.7 0.08 18.3 5.7 4.9 5.2 35.3 2
689 219769 Vitellogenin fused with SOD 2.2 0.04 1.9 0.06 17 38.1 223 5.7 6.5 12.9 25
Spot ID Gene ID Function R P R P SP Mre Mrp pIe pIp SC (%) NP
  24 h 48 h  
520 300012 Actin and related proteins 0.2 0.00 0.3 0.00 62.6 41.8 5.5 5.3 30.0 8
550 300012 Actin and related proteins 1.8 0.03 1.8 0.01 60.5 41.8 5.3 5.3 14.3 4
698 300012 Actin and related proteins 1.8 0.01 2.0 0.00 36.2 41.8 5.1 5.3 22.8 8
752 300012 Actin and related proteins 4.4 0.00 3.5 0.03 28.2 41.8 5.6 5.3 36.3 17
724 305550 Actin and related proteins 1.7 0.06 2.2 0.02 32.0 41.8 5.7 5.3 37.9 12
749 305550 Actin and related proteins 3.7 0.00 1.9 0.10 29.1 41.8 5.5 5.3 28.9 10
417 100611 α-Tubulin 0.6 0.02 0.7 0.04 82.3 46.1 5.4 5.0 23.8 8
405 301837 α-Tubulin 0.6 0.03 0.9 0.45 81.9 49.9 5.3 5.0 33.0 16
757 220693 Arginine kinase 2.5 0.01 1.7 0.06 28.8 39.9 5.4 5.5 36.3 9
796 220693 Arginine kinase 2.1 0.03 1.6 0.08 24.4 39.9 5.1 5.5 34.9 10
438 300845 β-Tubulin 0.6 0.01 0.9 0.72 78.8 50.1 5.1 4.7 19.9 10
463 210624 Calreticulin 0.7 0.03 0.6 0.02 16 72.4 46.6 4.7 4.5 48.3 18
383 301074 Chaperonin ATPase, Cpn60/Hsp60p 3.8 0.01 2.9 0.00 89.1 61.4 5.6 5.8 31.2 22
809 301844 Enolase 1.9 0.00 1.4 0.42 26.1 46.8 6.0 5.7 28.1 8
1027 301844 Enolase 1.9 0.04 1.9 0.26 5.0 46.8 6.6 5.7 19.8 6
609 304126 FOG, leucine-rich repeat 1.5 0.07 2.9 0.01 22 47.2 42.1 4.6 4.7 18.6 7
801 303282 Glutathione transferase 1.8 0.01 1.4 0.12 24.2 24.8 5.3 5.4 36.8 12
784 305501 Glutathione transferase 1.3 0.16 2.5 0.04 25.3 25.2 5.8 5.6 36.2 10
794 317266 Glutathione transferase 1.7 0.01 1.3 0.27 25.0 25 5.4 5.2 67.3 18
474 230437 Glycoside hydrolase, family 9 0.2 0.02 0.4 0.02 18 71.5 49.1 5.3 5.2 18.2 6
441 309746 H+-transporting two-sector ATPase 0.5 0.03 0.8 0.34 78.1 56.8 5.2 5.5 43.8 16
779 309746 H+-transporting two-sector ATPase 1.1 0.73 3.0 0.03 26.5 56.8 5.9 5.5 64.2 20
641 251885 Serine endopeptidase 0.9 0.65 0.4 0.03 19 41.2 39.6 4.5 5.0 12.7 6
845 9558 Ubiquitin and ubiquitin-like proteins 0.7 0.32 0.6 0.04 18.3 5.7 4.9 5.2 35.3 2
301 219769 Vitellogenin fused with SOD 1.6 0.04 2.2 0.03 17 107.0 223 5.6 6.5 13.3 34
322 219769 Vitellogenin fused with SOD 2.3 0.03 2.8 0.03 17 102.6 223 5.6 6.5 12.3 21
325 219769 Vitellogenin fused with SOD 2.5 0.01 2.6 0.04 17 101.9 223 5.6 6.5 12.6 29
576 219769 Vitellogenin fused with SOD 1.7 0.15 2.8 0.01 17 51.9 223 5.7 6.5 7.5 18
580 219769 Vitellogenin fused with SOD 5.1 0.05 2.9 0.01 17 51.8 223 5.6 6.5 9.9 22
660 219769 Vitellogenin fused with SOD 1.1 0.70 2.4 0.00 17 40.0 223 5.3 6.5 12.8 26
1545 219769 Vitellogenin fused with SOD 4.7 0.00 5.0 0.00 17 51.8 223 5.6 6.5 9.9 22
  1. D. pulex clone G on top, D. pulex clone M at the bottom. From left to right: Spot ID, gene ID, protein function, expression ratios (R) contrasting stress (24 h or 48 h under starvation, 20 °C) and control (20 °C, ad libitum feeding) conditions and associated P values (t-tests), predicted length of N-terminal signal peptides (SP), experimental and predicted molecular masses (Mre, Mrp) and isoelectric points (pIe, pIp) of the mature protein, sequence coverage (SC), and number of matching peptides (NP). Bold R and P values indicate statistical significance. See Table 3 for details. Number of gels (biological replicates, 25-30 animals each) for clone G or M: control (5 or 4) and 24 h (4 or 4) or 48 h (4 or 5) of stress