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Fig. 3 | BMC Genomics

Fig. 3

From: Characterization of microRNA and mRNA expression profiles in skin tissue between early-feathering and late-feathering chickens

Fig. 3

Validation of miRNA and mRNA expression profiles by qPCR. a The expression pattern of five genes were tested between L2 and N1 group. b The expression pattern of five genes were tested between F1 and N1 group. c The expression pattern of five miRNAs were tested between L2 and N1 group. d The expression pattern of five miRNAs were tested between F1 and N1 group. U6 and β-actin were used as reference genes for miRNA and mRNA testing, respectively. Fold change values of qPCR were calculated using the comparative 2–∆∆CT (∆∆Ct = ∆Ct (target gene) – ∆Ct (reference gene)) from at least three independent experiments. Fold change values of sequencing data were calculated using the DESeq2 system

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BMC Genomics

ISSN: 1471-2164

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