Fig. 5

Aberrant methylation in the upstream regions of ZIC3 and NR2F2, visualized in UCSC genome browser. DMRs are indicated by light blue bar; methylated levels in the twins are showed in blue (D3) and red (D4) bars. Transcription factor binding sites are also showed in zooming-in panels, which are indicated by black bars with names marked in front. An arrow gives TSS and transcriptional orientation. Two genes, ZIC3 (a) and NR2F2 (b), are showing differentially methylated in the upstream of TSS, and these two genes are known be associated with CHD. Transcription factor binding sites analyses are performed by ChIP-seq data of RNA Pol II and TBP derived from ENCODE. Bisulfite sequencing data (c and d) show methylation status of the same region of ZIC3 and NR2F2 as in (a) and (b). Methylated and unmethylated CpG sites are shown as black and white circles, respectively