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Fig. 5 | BMC Genomics

Fig. 5

From: Intragenic sequences in the trophectoderm harbour the greatest proportion of methylation errors in day 17 bovine conceptuses generated using assisted reproductive technologies

Fig. 5

Pyrosequencing analysis of genes with differentially methylated gene bodies. Four genes identified as having differentially methylated intragenic regions by the EDMA analysis were selected for pyrosequencing. All assays confirmed the directionality of the change in methylation at these loci between control samples and ART samples (ae). DNA methylation was significantly lower in day 17 SOV TE samples, relative to in vivo controls (b).

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