Accurate diagnosis of spinal muscular atrophy and 22q11.2 deletion syndrome using limited deoxynucleotide triphosphates and high-resolution melting

Table 1 Primer Sequences and Data for Reference and Target Genes

Chr.	Region	Primer-Forward	Primer-Reverse	Amplicon Size (bp)	Amplicon Tm (°C)
7	CFTR exon 7	TTGTGATTACCTCAGAAATGATTGA	CATTGCTTCTTCCCAGCAGT	68	77.5
5	SMN1 exon 7	TTCCTTTATTTTCCTTACAGGGTTT	CCTTCCTTCTTTTTGATTTTGTCTG	50	73.5
22	CLTCL1	TCAGCTCCTCCAGCTCATCT	TGCATGGATGGACAAGAGTT	82	85
22	KLHL22	CTCTCGTTCCGGTGGTACAT	TGATGGAAGCTGAGGTCCTG	100	90
22	PI4KA	GCCTGTGGGAGGACAAAATA	TTCTGGCACACCAGTTCATC	97	83

ISSN: 1471-2164