Fig. 7

Allelic integration of RNA-, ChIP-seq and WGBS datasets from human brain. a Analysis of allele-specific gene expression using RNA-seq data from adult human brain. Imprinted genes are highlighted in red and monoallelically expressed genes (defined by total expression (RPKM > 1), allele-specific coverage (mapped reads > 100) and expression bias (> 90% of transcript levels from one allele)) are highlighted in blue and orange. MEST, an imprinted gene, is highly expressed in brain and shows the expected allelic bias. b UCSC genome browser screenshot of the MEST locus showing allele-agnostic (total) and allele-specific (blue and red) DNAme levels in adult brain. DNAme levels in gametes (oocyte & spermatozoa) are also shown [49]. RNA-seq and H3K27ac ChIP-seq data from human brain were integrated using MEA and allele-agnostic (total) as well as allele-specific coverage is shown for each. Note that only the expressed allele, haplotype 2 (hap2) is unmethylated and enriched for H3K27ac. Also see Additional file 2: Table S2