Fig. 3
Tree of the TnpAY1 representative sequences. The tree is rooted to the branch that separates class 1 and 2. Color code for the bootstrap value (b) intervals: 0.7 < b ≤ 0.8 in green, 0.8 < b ≤ 0.9 in blue and 0.9 < b ≤ 1 in red. The MCL subclasses obtained with IF = 3.0 are colored and subclass numbers are added in the corresponding segments. Based on the tree topology, subclass 2.1 can be further divided in groups 2.1.1 and 2.1.2 and three subsets of sequences appear misclassified by MCL (highlighted by black and grey segments) according to their tree location. The taxonomic origin of the sequences is shown as a colored code in the outer ring (NCBI taxonomy phyla level). The phyla with less than three sequences on the tree are not colored. The Phyla are color-coded at the bottom of the figure. The tree display was obtained with online iTOL [83] (https://itol.embl.de/)