Fig. 1
a Schematic representation of the pVJ-neo and pHJH4 reporter systems. b Checking of pVJ-neo and pHJH4 reporter systems in TSA plates. The same amount of spores (106) of S. coelicolor strains containing pVJ-neo, pHJH4, pRL-neo and no vector were inoculated in TSA kanamycin cultures (50 μg per mL) with or without 5 μg per mL of vancomycin. Plates were analysed after 4 days of growth at 30 °C. c-d Induction of pVJ-neo using 96-well microplates. Different concentrations of vancomycin (0, 1, 5, 10 and 20 μg per mL) were added to TSA containing either no kanamycin (c) or 50 μg per mL of kanamycin (d). In each well was added 106 spores of S. coelicolor W1. Plates were then incubated at 30 °C during 52 h. Growth was determined by optical density (420 nm) using a BMG Fluostar Optima fluorometer. (e-f) S. coelicolor W1 cells were grown during 6 days in Difco nutrient agar containing kanamycin (50 μg per mL) and vancomycin (50 μg per mL, upper panels) / (0.5 μg per mL, lower panels) and with (f) or without 1% K2HPO4 (e) addition. Three different amount of spores (105, 106 and 107) were inoculated in each case. g-h Same as e-f but with the addition of teicoplanin (5 μg per mL) instead of kanamycin