Fig. 2
a Selection of mutant strains inducing vanJp in high Pi concentrations. 108 spores of S. coelicolor W1 and W2 strains were streaked in TSA containing kanamycin (50 μg per mL) and vancomycin (50 or 0.5 μg per mL). Plates were grown at 30 °C until mutant colonies could be observed. b Isolation of “L” mutants after increasing the amount of the inoculum (i.e. 109 spores) and repeating the experiment several times. In the figure is shown the isolation of the L5 mutant as an example. c Scheme of the “H” and “L” mutants isolated and analysed in this study. d Scheme representing the four main growth patterns observed for the strains in the growth conditions of the study, which are summarized with the next symbols (−, +, ++ and ++++). e-f Analysis of the growth of the mutant strains incubated at 30 °C during 4 days in: (e) DifcoNA with 1% K2HPO4 and 50 μg per mL of vancomycin or in (f) DifcoNA with 1% K2HPO4 and 5 μg per mL of teicoplanin