Fig. 4

a Schematic representation of the localization of the mutation identified in L1 and L5 strains according to a predicted structure of the S. coelicolor VanS protein by Hutchings and co-workers [18]. b Table summarising the results of L1, L5, WT and complemented strains according to their capability to grow in teicoplanin containing plates without the presence of the inducer (i.e. vancomycin). c Plate showing the growth of the developed vanS strains in Difco nutrient agar with 5 μg per mL of teicoplanin (and no vancomycin) after 6 days of incubation at 30 °C. d Genetic organization of murT (green) gatD (blue) in S. coelicolor. The localization of the mutation of L2 in SCO1213 is shown with a vertical line. In the image is also shown the conserved domains detected for the proteins according to the NCBI’s conserved domain database [28]. e Red overproduction phenotype in the spore stocks of L1 and L5 in comparison to the rest of strains. In the picture, the spore stock of L5 is shown at the left and the spore stock of W2 at the right