Table 2 Sanger and Illumina sequencing analyses of the “H” and “L” mutant strains. The table shows the mutations identified by both Illumina and Sanger sequencing analyses in each of the mutant strains
Mutation in chromosomal van genes | Mutation in vanSR of pVJ | Mutation in other loci | |
|---|---|---|---|
W1 | No | No | No |
W2 | No | – | No |
H1 | No | No | No |
H2 | No | No | ? |
H3 | No | No | ? |
H4 | No | No | No |
H5 | No | No | SCO4474 Thr477- Lys477 |
H6 | No | No | ? |
H7 | No | No | ? |
H8 | No | No | ? |
H9 | No | No | ? |
H10 | No | No | ? |
H11 | No | No | SCO3167 (5nt-insertion) |
H12 | No | No | No |
L1 | VanS Gly77-Asp77 | No | No |
L2 | No | No | SCO1212 Val120-Gly120 |
L4 | No | – | SCO1212 (GTG→GTT) |
L5 | VanS Gly77-Asp77 | – | No |