Table 3 Strains and plasmids used in this work

Bacterial strains

Description

Reference

 E. coli DH5α

E. coli standard strain; F′Ф80 LacZ ∆M15

[29]

 E. coli ET12567 (pUZ8002)

E. coli conjugative strain; dam, dcm, hsdS, cat, tet

[30]

 S. coelicolor M145

S. coelicolor parental strain; SCP1⁻ SCP2⁻

[22]

 S. coelicolor W1

S. coelicolor M145 (pVJ-neo); Hyg^R

This work

 S. coelicolor W2

S. coelicolor M145 (pHJH4); Apra^R

This work

 S. coelicolor RL-neo

S. coelicolor M145 (pRL-neo); Hyg^R

This work

 S. coelicolor H1-H12

S. coelicolor M145 (pVJ-neo) suppressor strains; Hyg^R

This work

 S. coelicolor L1

S. coelicolor M145 (pVJ-neo) suppressor strain; Hyg^R

This work

 S. coelicolor L2

S. coelicolor M145 (pVJ-neo) suppressor strain; Hyg^R

This work

 S. coelicolor L4

S. coelicolor M145 (pHJH4) suppressor strain; Apra^R

This work

 S. coelicolor L5

S. coelicolor M145 (pHJH4) suppressor strain; Apra^R

This work

 S. coelicolor L5-S

S. coelicolor L5 (pVJ); Apra^R, Hyg^R

This work

 S. coelicolor L5-S*

S. coelicolor L5 (pVJc); Apra^R, Hyg^R

This work

 S. coelicolor WT-S*

S. coelicolor M145 (pVJc-neo); Hyg^R

This work

 S. coelicolor L2–1213

S. coelicolor L2 (pCOM-1213-L2); Apra^R, Hyg^R

This work

 S. coelicolor L4–1213

S. coelicolor L4 (pCOM-1213-L4); Apra^R, Hyg^R

This work

 S. coelicolor L2-Con

S. coelicolor L2 (pSET152-SCO1212); Apra^R, Hyg^R

This work

 S. coelicolor L4-Con

S. coelicolor L4 (pMS82-SCO1212); Apra^R, Hyg^R

This work

 S. coelicolor M145-pVJ-1212

S. coelicolor M145 (pVJ-1212); Hyg^R

This work

 S. coelicolor M145-pVJ-1213

S. coelicolor M145 (pVJ-1213); Hyg^R

This work

 S. coelicolor M145-pVJc-1212

S. coelicolor M145 (pVJc-1212); Hyg^R

This work

 S. coelicolor M145-pVJc-1213

S. coelicolor M145 (pVJc-1213); Hyg^R

This work

Plasmids

Description

Reference

 pMS82

Integrative vector; phiBT1; Hyg^R

[31]

 pSET152

Integrative vector; phiC31; Apra^R

[32]

 pHJH4

Integrative vector containing vanJp-neo; phiC31; Apra^R

[13]

 pVJ

vanRS-vanJ_prom cloned in pMS82; Hyg^R

This work

 pVJ-neo

Promoterless neo cloned in pVJ; Hyg^R

This work

 pVJc

L5 vanRS-vanJ_prom cloned in pMS82; Hyg^R

This work

 pVJc-neo

Promoterless neo cloned in pVJc; Hyg^R

This work

 pRL-neo

rpsL_prom cloned in pVJ-neo; Hyg^R

This work

 pCOM-1213-L2

SCO1212–13 cloned in pSET152; Apra^R

This work

 pCOM-1213-L4

SCO1212–13 cloned in pMS82; Hyg^R

This work

 pSET152-SCO1212

SCO1212 cloned in pSET152; Apra^R

This work

 pMS82-SCO1212

SCO1212 cloned in pMS82; Hyg^R

This work

 pVJ-1212

Promoterless SCO1212 cloned in pVJ; Hyg^R

This work

 pVJ-1213

Promoterless SCO1213 cloned in pVJ; Hyg^R

This work

 pVJc-1212

Promoterless SCO1212 cloned in pVJc; Hyg^R

This work

 pVJc-1213

Promoterless SCO1213 cloned in pVJc; Hyg^R

This work