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Table 1 Contigs with greater than 10-fold (K326/TI 1406) or 7-fold (K326/ K326-va) differential expression

From: Characterization of Nicotiana tabacum genotypes possessing deletion mutations that affect potyvirus resistance and the production of trichome exudates

Contig ID

K326 coverage

K326/TI 1406

K326/K326 va

PCR

Annotation

S or T

24507c0g1i1

27.7

na

0.65

yes

Cembratrienol synthase 3

S

33989c1g1i3

408.3

2351.50

0.18

–

Cembratrienol synthase 3

S

33989c1g1i6

323.3

1443.83

0.06

yes

Cembratrienol synthase 2a

S

33989c1g1i4

204.4

1333.67

0.11

yes

Cembratrienol synthase 2a

S

33989c1g1i5

204.9

952.75

0.14

yes

Cembratrienol synthase 2a

S

33989c1g1i2

221.1

463.05

0.10

yes

Cembratrienol synthase 2a

S

8697c0g1i1

83.2

447.40

158.00

–

Uncharacterized protein LOC104224958

S

87014c0g1i1

59.5

203.87

−0.08

–

–

 

23542c0g2i2

31.1

183.15

0.20

–

–

 

22222c0g1i1

296.4

127.47

29.46

–

Auxin-repressed 12.5 kDa protein-like

S

33989c1g2i1

141.4

113.38

0.36

yes

Cembratrienol synthase 2a

S

22534c0g3i1

21.2

79.41

29.55

yes*

Uncharacterized protein LOC104232912

S

35943c0g2i1

24.3

48.72

35.73

yes*

3-Isopropylmalate dehydratase

S

23466c1g2i1

656.1

47.47

−0.39

yes

Sar8.2c

T

2175c0g2i1

55.9

42.48

24.70

–

Trehalose-phosphate phosphatase G

S

22475c0g1i2

56.0

36.96

18.54

yes*

Cyclic phosphodiesterase-like

S

2175c0g1i1

56.6

33.51

22.45

–

Trehalose-phosphate phosphatase G

S

33215c0g1i1

75.0

33.11

0.18

–

Uncharacterized protein LOC107810047

 

64746c0g1i1

22.7

32.65

327.11

–

–

 

28889c6g4i1

34.6

30.27

0.40

–

Uncharacterized protein LOC107777218

T

42740c0g1i1

40.8

25.89

0.24

yes

Uncharacterized protein LOC107765064

T

27218c1g1i1

10.3

25.31

0.30

–

Uncharacterized protein LOC107786237

S

32651c2g7i3

10.8

25.24

−0.18

–

F-box protein

T

65564c0g1i1

29.0

23.74

0.05

–

–

 

23466c1g2i2

966.4

23.70

−0.36

yes

Sar8.2 k

T

25192c0g1i1

34.1

22.46

0.34

–

Bifunctional purple acid phosphatase 26

S

25436c0g1i2

19.4

22.33

0.22

yes

Uncharacterized protein LOC104238501

S

8352c0g1i1

10.9

21.95

0.79

–

Uncharacterized protein LOC107812316

T

25661c1g1i3

42.9

19.09

11.64

yes*

Cyclic phosphodiesterase

S

40439c0g1i1

10.7

18.95

0.19

–

–

 

22779c0g1i1

118.1

18.95

17.29

–

heat- and acid-stable phosphoprotein

S

86871c0g1i1

18.4

17.78

0.73

yes*

Uncharacterized protein LOC104224418

S

20225c0g1i1

28.8

17.76

0.18

yes

–

 

22899c0g2i1

53.5

17.74

0.28

–

Uncharacterized protein LOC107760627

T

21258c0g1i2

61.9

17.72

0.02

–

Nucleoside diphosphate kinase 3

S

75249c0g1i1

44.0

16.17

−0.23

–

Uncharacterized protein LOC107794475

S

18187c0g2i2

17.8

15.68

0.11

yes

Uncharacterized protein LOC104232736

S

64896c0g1i1

10.3

14.35

0.36

–

–

 

26833c0g2i1

24.0

14.24

11.16

–

Ethylene-responsive transcript. Factor 4

S

16240c0g1i1

26.9

14.05

0.05

yes

4-coumarate--CoA ligase

S

25958c0g1i2

17.8

12.81

7.69

yes*

Heat stress transcription factor A

S

65623c0g1i1

24.4

12.78

0.59

–

–

 

19615c0g1i2

10.6

12.48

0.58

–

Interaptin

T

3660c0g2i1

10.3

12.47

−0.02

–

Diaminopimelate epimerase,

S

30135c0g1i1

15.0

12.36

1.03

–

Cytochrome P450 78A5

S

3660c0g1i1

11.8

12.26

0.10

–

Diaminopimelate epimerase,

S

30979c0g1i5

50.1

12.23

1.19

–

GDSL esterase/lipase

S

33333c2g1i1

18.7

11.97

−0.05

–

–

 

12543c0g1i1

35.3

11.85

0.28

–

Uncharacterized protein LOC104210975

S

28570c0g1i1

42.9

11.57

0.04

yes

Protein disulfide-isomerase

S

85964c0g1i1

10.5

11.00

0.20

yes*

Heat shock protein 83

S

23580c0g1i1

104.5

11.00

−0.33

yes

Early light-induced protein 1, chloroplastic

S

18777c2g1i1

11.4

10.71

0.56

–

Uncharacterized protein LOC107823745

S

26611c0g1i2

29.2

10.55

−0.18

yes

–

 

32166c0g3i1

25.4

10.45

0.37

–

Uncharacterized protein LOC107771105

T

  1. Columns 3 and 4 are expressed in terms of expression fold change. Column 5 shows contigs which failed to amplify using PCR in TI 1406; those that also failed to amplify in K326-va and TN90 are indicated with an asterisk. Column 7 shows the best matching ancestral tobacco species (S = N. sylvestris, T = N. tomentosiformis)