Fig. 4

The organization of the actin cytoskeleton is initially perturbed but is resolved later in the par-4 mutant dauer germline. Daf-2(e1370) dauer larvae that express a GFP reporter that marks the actin cytoskeleton were examined to monitor any change to the actin cytoskeletal network in the germ line. All images in the left panel are merged, condensed Z stacks. Actin filaments are organized at the membrane adjacent to the rachis in the daf-2 dauer germ line (arrow) at 48 h after shifting to the restrictive temperature of 25 °C. Actin organization is perturbed (arrowhead) in the germ line of par-4 dauer larvae at 48 h, but is resolved at 72 h (arrow). Similarly, par-3 compromised dauer larvae exhibit defects in actin organization at the rachis-adjacent membrane. No defects were observed in the actin organization at the rachis-adjacent membrane in the daf-2; aak-1; aak-2(RNAi) treated dauer germ line at 48 h (arrow). n = 15, scale bar: 4 μm