Fig. 1

Pipeline for in silico characterization of candidate secreted effector proteins (CSEPs). The prediction of the secretoma was performed from the putative proteome. Effector candidates were identified from these secreted proteins that meet at least one of the following criteria: (I) Proteins with nuclear localization signal (NLS), (II) small (<= 150 aa) and cysteine (> 3%) (SCR) proteins, and (III) proteins containing repeats (PCR). They were also predicted by the EffectorP software. The set of CSEPs was formed with the sum of the proteins with NLS, SCR, and RCP and deduced by EffectorP (Pipeline adapted from Toro and Brachmann [38]). Proteins predicted more than once by the established criteria were counted only once. MrPeru - Moniliophthora roreri isolate from Peruvian subpopulation. Mp4145 and Mp1441 - M. perniciosa isolates from cacao subpopulations in Bahia. Mp4124 - M. perniciosa isolate from cacao subpopulation in Ecuador. Mp178 - M. perniciosa isolate from wild solanaceous subpopulation (Lobeira) in Minas Gerais. Mp4071 - M. perniciosa isolate from wild solanaceous subpopulation (Caiçara) in Bahia