Fig. 4
From: Novel long-range regulatory mechanisms controlling PKD2 gene expression
Several DHS regions show enhancer activities. a Alignment of DNAse I hypersensitivity data from A549 cells over the schematic linear representation of the region. Gene orientation is indicated with arrows. The cloned DHS elements are indicated with blue boxes at the top and identify open chromatin sites. b A549 cells were transfected with pGL3B luciferase reporter constructs containing the PKD2 basal promoter (PPKD2; 1169 bp) and fragments of DHS elements of the region A to H. Luciferase data are shown relative to the PKD2 basal promoter vector (=1). Error bars represent SEM (n = 9), * P < 0.0001 using unpaired t-tests