Table 3 Enzymes involved in the DHA biosynthesis of the FAS pathway
Enzymes | EC No. | KO | Mn4 | SW8 | Aurli | SchiM | Schag | Aplke | Phatr | Yarli | |
|---|---|---|---|---|---|---|---|---|---|---|---|
elongase | E1 | 2.3.1.199 | K10203 | 2 | 2 | 2 | 2 | 3 | 1 | 2 | 0 |
K10246 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | |||
E2 | 1.1.1.330 | K10251 | 3 | 3 | 3 | 5 | 2 | 6 | 9 | 1 | |
1.1.1.100 | K00059 | 87 | 87 | 89 | 75 | 78 | 102 | 63 | 47 | ||
E3 | 4.2.1.134 | K10703 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | |
K18880 | 2 | 2 | 2 | 2 | 2 | 7 | 0 | 5 | |||
E4 | 1.3.1.93 | K10258 | 4 | 3 | 3 | 2 | 4 | 8 | 3 | 2 | |
1.3.1.38 | K07753 | 2 | 2 | 2 | 3 | 0 | 3 | 2 | 3 | ||
desaturase | â–³9 | 1.14.19.1 | K00507 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
1.14.19.2 1.14.19.11 1.14.19.26 | K03921 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | ||
1.14.19.2 | K03922 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
â–³12 | 1.14.19.23 1.14.19.13 | K10255 | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 1 | |
1.14.19.6 1.14.19.22 | K10256 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | ||
n-3 | 1.14.19.25 1.14.19.35 1.14.19.36 | K10257 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | |
1.14.19.13 | K21707 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
â–³6 | 1.14.19.2 | K00508 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
1.14.19.3 | K10226 | 17 | 14 | 16 | 13 | 16 | 18 | 5 | 3 | ||
â–³8 | 1.14.19.4 | K13076 | 8 | 9 | 9 | 9 | 13 | 6 | 4 | 4 | |
K21732 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
â–³5 | 1.14.19.44 | K10224 | 1 | 1 | 0 | 2 | 1 | 3 | 0 | 0 | |
â–³4 | 1.14.19.31 | K12418 | 9 | 2 | 2 | 5 | 8 | 11 | 4 | 0 | |
peroxisomal β-oxidation | P1 | 1.3.3.6 | K00232 | 19 | 20 | 19 | 19 | 16 | 24 | 7 | 10 |
P2 | 4.2.1.17 | K01825 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | |
K01782 | 15 | 17 | 16 | 16 | 12 | 21 | 6 | 3 | |||
K07515 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | |||
P3 | 1.1.1.211 | ||||||||||
K10527 | 11 | 13 | 11 | 11 | 12 | 15 | 5 | 3 | |||
P4 | 2.3.1.16 | K07513 | 11 | 12 | 11 | 12 | 8 | 14 | 6 | 4 | |
long-chain fatty-acyl-CoA hydrolase | 3.1.2.2 | K01068 | 8 | 6 | 5 | 7 | 4 | 2 | 0 | 1 | |