Fig. 2

Read length and offset selection. In an ideal case scenario, the 3-nucleotide periodicity determines if the footprint length is coding (d), the peaks over start (a) and the last codon before stop (b) codons are used to calibrate offsets and most of the reads map to the first reading frame (c). Here, the plots demonstrate length 28 in human ribosome profiling sample (SRR493747, [15]). For more plots and datasets see Additional file 1