Table 1 Oligonucleotides used in this study
Primer target and designation | Primer sequence | Product size (bp) |
|---|---|---|
vaa genea | ||
VaaPG21-FE | 5’-AATGGGATAGTTAGTAAAGTCGG-3’ | 1983 |
VaaPG21-RE | 5’-AACCCTACCATTGTCGCTAAGA-3’ | |
VaaPG21-FE-IRD700 | 5’-IRD700-AATGGGATAGTTAGTAAAGTCGG-3’ | 1692 |
VaaPG21-RI-IRD800 | 5’-IRD800-GAAAAATCTCCTCGTGCTGATG-3’ | |
oppA gene | ||
OppAPG21-FE | 5’-GACAGTGGAAAAGCAGACAC-3’ | 1524 |
OppAPG21-RE | 5’-AGGAACTAAAATGTCCGGGT-3’ | |
OppAPG21-FI-IRD700 | 5’-IRDye700- CATCTTCTGGCCAAGCAACT − 3’ | 1144 |
OppAPG21-RI-IRD800 | 5’-IRDye800-CAGTCTTGGTATGAATCAAC-3’ | |