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Table 4 Selected miRNAs in oEVs in Stage 1 and 4 and associated pathways and target genes

From: Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo

miRNAs KEGG pathway p-value Examples Target Genes (Gene Symbol)
hsa-mir-10b-5p Pyrimidine metabolism 0.0214 NME4,NME2,POLR2D,POLR2A,POLR1B,NME1,CMPK1
hsa-miR-423-3p Fatty acid biosynthesis 0.0000 FASN, ACACA
Fatty acid metabolism 0.0000 FASN, ACACA, ACOX3, FADS2
hsa-miR-449a Carbon metabolism 0.0286 TKT,GAPDH,GOT1,H6PD,MDH2,RPIA,ENO1,ALDOA,ADH5
HIF-1 signaling pathway 0.0390 NFKB1,GAPDH,LTBR,BCL2,ENO1,LDHA,PIK3CA,SLC2A1,MKNK2,ALDOA,SERPINE1
hsa-miR-375 Hippo signaling pathway 0.0000 YAP1,YWHAG,CCND2,MOB1B,YWHAB,WWC1,AMOT,FZD8,MPP5,FZD4,DLG4,
Amino sugar and nucleotide sugar metabolism 0.0390 PGM2,CMAS,UXS1,UGP2,HK2
hsa-miR-24-3p Fatty acid biosynthesis 0.0000 FASN,MCAT,ACACA
Vitamin B6 metabolism 0.0001 PDXK,PNPO,PHOSPHO2
Endocytosis 0.0005 RNF41,PSD4,CHMP7,VPS45,SMAD2,CBL,DNM2,FGFR3,VTA1,AGAP3,CAV1,AP2B1,N
Hippo signaling pathway 0.0014 ACTB,GSK3B,FZD5,YWHAH,SMAD2,BTRC,APC,NF2,YWHAG,SMAD3,WWTR1,TGFB1,
Bacterial invasion of epithelial cells 0.0078 ACTB,ITGB1,CBL,DNM2,PIK3CB,CAV1,PXN,BCAR1,CLTC,CD2AP,CAV2,CTTN,PTK2,
TGF-beta signaling pathway 0.0228 SMAD2,ACVR1B,SMAD3,TGFB1,SKP1,MYC,EP300,IFNG,E2F4,CREBBP,TGFBR2,BMP4,
Glycosaminoglycan biosynthesis - heparan sulfate / heparin 0.0343 HS6ST2,NDST1,HS6ST1,EXTL3,HS2ST1,HS6ST3
DNA replication 0.0343 CNA,FEN1,MCM4,MCM5,MCM7,RNASEH1,PRIM1,POLA1
RNA degradation 0.0423 PDCP1A,CNOT6,TOB2,CNOT4,DDX6,HSPD1,EXOSC3,EXOSC1,BTG2,DCP2,RQCD1,
hsa-miR-429 Axon guidance 0.0009 PAK2,PPP3R1,RHOA,KRAS,PPP3CA,PTK2,CFL2,RAC1,LIMK1,EFNA1
Fc gamma R-mediated phagocytosis 0.0350 CRKL,CRK,WASF3,PLCG1,CFL2,RAC1,LIMK1,ARF6
Steroid biosynthesis 0.0000 SC5D,MSMO1,DHCR24,LSS,FDFT1,LIPA
Antigen processing and presentation 0.0019 B2M,IFI30,HSPA4,HSP90AA1,HLA-C,HLA-B,NFYA,PSME2,CANX,PDIA3,HSPA8,
Progesterone-mediated oocyte maturation 0.0039 ADCY1,CCNB1,PGR,ADCY7,CCNA2,CDC25B,CPEB4,HSP90AA1,PDE3A,RAF1,
Gap junction 0.0346 ADCY1,ADCY7,GNAS,MAP2K5,RAF1,TUBB,TUBB6,CDK1,KRAS,TUBA8,GNA11
hsa-miR-34b-3p Glycosaminoglycan degradation 0.0000 GNS,IDS,GLB1
hsa-miR-200b-3p Ras signaling pathway 0.0043 KSR1,RASA2,SHC1,PAK2,ETS1,RALBP1,PLD1,RHOA,KRAS,PLCG1,REL,FLT1,
Neurotrophin signaling pathway 0.0273 CRKL,CRK,SHC1,FRS2,BCL2,RHOA,KRAS,RPS6KA5,PLCG1,JUN,KIDINS220
hsa-miR-92a-3p Cell cycle 0.0000 GSK3B,PCNA,SMC1A,CCNB1,SMAD2,HDAC1,CCND2,DBF4,SMC3,CDKN1B,
Adherens junction 0.0000 ACTB,TGFBR1,WASL,SMAD2,IQGAP1,IGF1R,VCL,FYN,MLLT4,NLK,CDH1,S
Thyroid hormone signaling pathway 0.0011 ACTB,GSK3B,PRKCA,MED13L,NRAS,RCAN1,PIK3CB,HDAC1,MED13,NOTCH2
FoxO signaling pathway 0.0024 IRS2,BRAF,STAT3,TGFBR1,CCNB1,SMAD2,NRAS,STK4,GABARAPL2,PIK3CB,SETD7
RNA transport 0.0025 SAP18,GEMIN6,NUP153,TGS1,NUP210,XPOT,EIF3B,ELAC2,RPP14,EIF2S3,RNPS1
p53 signaling pathway 0.0045 CCNB1,BID,THBS1,CCND2,CDK1,GADD45A,CDK6,CHEK1,ATM,CCND1,CCNE2,SHISA5
Signaling pathways regulating pluripotency of stem cells 0.0178 PCGF6,JARID2,TCF3,GSK3B,STAT3,KAT6A,SMAD2,NRAS,INHBB,PIK3CB,WNT5A,TBX3
Protein processing in endoplasmic reticulum 0.0179 HSPA1A,EIF2AK1,RAD23B,ERO1L,NPLOC4,SEL1L,UBQLN1,SEC23A,SSR2,P4HB,XBP1
Notch signaling pathway 0.0190 HDAC1,CTBP1,DTX2,NOTCH2,HES1,HDAC2,KAT2A,RBPJ,KAT2B,EP300,CREBBP,MAML1
Spliceosome 0.0210 HSPA1A,LSM7,DDX23,SRSF5,PPIL1,U2SURP,EFTUD2,SF3B1,U2AF2,SNRPE,SF3A3
MAPK signaling pathway 0.0210 BRAF,DUSP4,PRKCA,HSPA1A,TGFBR1,NFKB1,NRAS,CRKL,STK4,ELK4,CRK,DUSP6
Wnt signaling pathway 0.0386 GSK3B,PRKCA,LRP6,TBL1X,BTRC,VANGL1,WNT5A,CTBP1,CCND2,FZD6,AXIN1,SKP1
Focal adhesion 0.0393 BRAF,ACTB,GSK3B,PRKCA,ITGB8,CRKL,CRK,THBS1,ITGA8,PIK3CB,PIP5K1C,PPP1CC
Biosynthesis of amino acids 0.0473 TKT,BCAT2,GAPDH,PHGDH,TALDO1,PKM,SHMT2,BCAT1,MTR,ENO1,GOT2,PFKM,ASS1
hsa-miR-151a-3p Biosynthesis of unsaturated fatty acids 0.0000 PTPLB,ELOVL5,SCD
Fatty acid metabolism 0.0003 HADH, PTPLB,ACACA,SCD,ELOVL5
hsa-miR-30d-5p Mucin type O-Glycan biosynthesis 0.0000 GALNT7,B4GALT5,ST3GAL1,GALNT6,GCNT3,GALNT1,GALNT3,GALNT2
Oocyte meiosis 0.0000 FBXO5,CAMK2D,PGR,BTRC,CALM1,CPEB4,YWHAG,PPP1CC,PPP3R1
Ubiquitin mediated proteolysis 0.0000 UBE2R2,BTRC,FBXW7,TRIM37,CUL2,NEDD4L,FZR1,PRPF19,MAP3K1
mRNA surveillance pathway 0.0317 MSI2,SAP18,PPP2R3A,EIF4A3,HBS1L,PPP1CC,CPSF2,CPSF6,PAPOLA
hsa-miR-125b-5p ErbB signaling pathway 0.0016 PRKCA,ERBB2,NRAS,CRKL,CRK,PAK2,RAF1,CDKN1B,EIF4EBP1,ERBB3
Regulation of actin cytoskeleton 0.0135 SSH2,EZR,NRAS,CRKL,CRK,PIP5K1C,PAK2,ARHGEF12,PPP1CC,ACTG1
  1. Table shows selected miRNAs based on differential expression and high abundance in EVs in Stage 1 and Stage 4 and associated representative pathways and target genes