CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

Table 1 Mendelian inheritance of eGFP expression in testcrosses

Cross^a	Parents	Hypothesized Genotypes			Phenotype^d
Cross^a	Parents	Parental (male x female)	Offspring^b	N^c	eGFP⁺/eGFP⁻	Goodness of fit
357	354–3 x 295–8	Eef1a ^{FLAG-P2A-eGFP/+} x Eef1a ^+/+	Eef1a ^{FLAG-P2A-eGFP/+} Eef1a^+/+ (1:1)	263	136/127	χ² = 0.647, p = 0.58
358	291–10 x 354–5	Eef1a ^+/+ x Eef1a ^{FLAG-P2A-eGFP/+}	Eef1a ^{FLAG-P2A-eGFP/+} Eef1a^+/+ (1:1)	124	71/53	χ² = 0.018, p = 0.11
359	354–3 x 354–5	Eef1a ^{FLAG-P2A-eGFP/+} x Eef1a ^{FLAG-P2A-eGFP/+}	EeF1a ^{FLAG-P2A-eGFP/FLAG-P2A-eGFP} Eef1a ^{FLAG-P2A-eGFP/+} Eef1a^+/+ (1:2:1)	111	81/30	χ² = 0.777, p = 0.62

^aAs described in Fig. 2a; ^bExpected ratio is indicated in parentheses. ^clarvae at 96 hpf; ^d eGFP^dim larvae were scored as eGFP⁻ in crosses 358 and 359