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Table 1 Mendelian inheritance of eGFP expression in testcrosses

From: CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

Crossa Parents Hypothesized Genotypes   Phenotyped  
Parental (male x female) Offspringb Nc eGFP+/eGFP Goodness of fit
357 354–3
x
295–8
Eef1a FLAG-P2A-eGFP/+
x
Eef1a +/+
Eef1a FLAG-P2A-eGFP/+
Eef1a+/+ (1:1)
263 136/127 χ2 = 0.647, p = 0.58
358 291–10
x
354–5
Eef1a +/+
x
Eef1a FLAG-P2A-eGFP/+
Eef1a FLAG-P2A-eGFP/+
Eef1a+/+ (1:1)
124 71/53 χ2 = 0.018, p = 0.11
359 354–3
x
354–5
Eef1a FLAG-P2A-eGFP/+
x
Eef1a FLAG-P2A-eGFP/+
EeF1a FLAG-P2A-eGFP/FLAG-P2A-eGFP
Eef1a FLAG-P2A-eGFP/+
Eef1a+/+ (1:2:1)
111 81/30 χ2 = 0.777, p = 0.62
  1. aAs described in Fig. 2a; bExpected ratio is indicated in parentheses. clarvae at 96 hpf; d eGFPdim larvae were scored as eGFP in crosses 358 and 359