Table 1 Primers used for mRNA levels measurement
From: DNA methylation of the promoter region of bnip3 and bnip3l genes induced by metabolic programming
Gene | Primer (5′ to 3′) | Tm in °C |
|---|---|---|
bnip3a | F: CCTGTGACAGTCCTCCGAGA | 60 |
R: CCACTTCACGTCTCCGTTCT | ||
bnip3b | F: GAGAACAACCCACCAAAGGA | 60 |
R: GTATATCCCCAGGCCAACTG | ||
bnip3la1 | F: CAAACTCCACCACACCCTCT | 60 |
R: CTGATCTGGACTGGGAGGTC | ||
bnip3la2 | F: GGAGAGTCAGGCCCCTCAG | 61 |
R: TCCTGATCTGGACTGGAAGG | ||
bnip3lb1 | F: GAACAACGGAGACGCTGGA | 61 |
R: GGTGGAGGTAGACTGGGACA | ||
bnip3lb2 | F: GCTGTATCAGAGAACAACGGACTA | 60 |
R: CATGCTGAGCGTCCAGTAGA |