Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates

Table 2 Sensitivity, specificity and predictive values of the multiplex protocol using different polymerases (N = 47)

Polymerase	FP	TP	TN	Sensitivity (CI 95%)	Specificity (CI 95%)	PPV (CI 95%)	NPV (CI 95%)
TaKaRa® Ex Taq Hot Start Version	12	35	0	100%	0%	74.47%	NA
TaKaRa® Ex Taq Hot Start Version	12	35	0	(90.1%. 100%)	(0.0%. 26.5%)	(59.7%. 86.1%)	(NA)
KAPA 2G Fast Multiplex PCR kit	12	35	0	100%	0%	74.47%	NA
KAPA 2G Fast Multiplex PCR kit	12	35	0	(90.1%. 100%)	(0.0%. 26.5%)	(59.7%. 86.1%)	(NA)
AmpliTaq® Gold DNA Polymerase	0	35	12	100%	100%	100%	100%
AmpliTaq® Gold DNA Polymerase	0	35	12	(90.1%. 100%)	(73.5%. 100%)	(90.1%. 100%)	(73.5%. 100%)

FP False positive results, FN False negative results, TP True positive results, TN True negative results, PPV Positive predictive value, NPV Negative predictive value, CI 95 95% confidence interval, NA Not available

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