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Table 5 Direct concordance and Kappa indices of the multiplex PCR touchdown protocol

From: Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates

Multiplex PCR approach genotyping technique (this study) Original genotyping technique [5]
Clade A1 Clade A2 Clade B1 Clade B2 Clade C1 Clade C2 Total
Clade A1 3 0 0 0 0 0 3
Clade A2 1 5 0 0 0 0 6
Clade B1 0 0 8 0 0 0 8
Clade B2 0 0 0 0 0 1 1
Clade C1 0 0 0 0 12 0 12
Clade C2 0 0 0 0 0 3 3
Total 4 5 8 0 12 4 33
Agreement Expected agreement Kappa index Std. Error of Kappa index Z p-value
0.939 0.240 0.920 0.0917 10.04 <  0.001