A comparative analysis of library prep approaches for sequencing low input translatome samples

BMC Genomics

Table 1 Duplication rate of libraries prepared by different kits

Kit	Sample type	PCR cycles for lib prep	Percent_Duplication (%)
			replicate			avg
			1	2	3	avg
NEB^a	Input	22	97	99	97	97
NEB^a	IP	22	99	99	99	99
NuG/Tru^b	Input	8	52	53	52	53
NuG/Tru^b	IP	8	36	31	26	31
SMTer^c	Input	15	83	86	85	85
SMTer^c	IP	15	86	83	68	79
Tru4^d	Input	22	67	77	71	72
Tru4^d	IP	22	85	82	99	89
Tru70^e	Input	19	90	94	92	92
Tru70^e	IP	19	98	97	96	97
SMTseq4^f	Input	11	40	38	40	40
SMTseq4^f	IP	11	60	49	48	52
SMTseq0.25^f	Input	11	37	37	41	38
SMTseq0.25^f	IP	11	59	50	47	52

ISSN: 1471-2164