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Table 1 Duplication rate of libraries prepared by different kits

From: A comparative analysis of library prep approaches for sequencing low input translatome samples

Kit Sample type PCR cycles for lib prep Percent_Duplication (%)
replicate avg
1 2 3
NEBa Input 22 97 99 97 97
IP 99 99 99 99
NuG/Trub Input 8 52 53 52 53
IP 36 31 26 31
SMTerc Input 15 83 86 85 85
IP 86 83 68 79
Tru4d Input 22 67 77 71 72
IP 85 82 99 89
Tru70e Input 19 90 94 92 92
IP 98 97 96 97
SMTseq4f Input 11 40 38 40 40
IP 60 49 48 52
SMTseq0.25f Input 11 37 37 41 38
IP 59 50 47 52
  1. aNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina
  2. bNugen Ovation® RNAseq System v2
  3. cSMARTer® Stranded Total RNA-Seq Kit-Pico Input Mammalian User
  4. dTruSeq® RNA sample preparation v2,4 ng
  5. eTruSeq® RNA sample preparation v2,70 ng
  6. fSMART-Seq v4 Ultra Low Input RNA Kit