A comparative analysis of library prep approaches for sequencing low input translatome samples

BMC Genomics

Table 2 Number of features with CPM > 0

Kit	Sample type	Number of features with coverage (CPM > 0)				Number of reads of all expressed features (CPM > 0)
		Replicate			avg	Replicate			avg
		1	2	3	avg	1	2	3	avg
NEB	Input	13,543	12,464	11,419	12,475	22,373,163	26,292,017	22,305,929	23,657,036
NEB	IP	11,059	12,165	14,331	12,518	16,668,355	27,323,849	15,122,257	19,704,820
NuG	Input	16,946	16,458	15,624	16,343	5,529,417	7,615,866	7,482,819	6,876,034
NuG	IP	17,715	17,841	18,198	17,918	3,223,153	5,319,712	5,141,329	4,561,398
SMTer	Input	12,281	10,184	10,929	11,131	759,221	762,562	750,159	757,314
SMTer	IP	12,422	11,168	15,684	13,091	701,059	645,414	464,199	603,557
Tru4	Input	19,906	19,863	19,631	19,800	35,368,451	45,466,334	31,299,550	37,378,112
Tru4	IP	17,467	18,899	21,161	19,176	36,785,680	28,571,125	18,031,264	27,796,023
Tru70	Input	15,957	15,577	16,082	15,872	32,103,296	33,797,108	25,797,283	30,565,896
Tru70	IP	14,430	15,213	15,680	15,108	31,150,532	29,799,544	27,902,766	29,617,614
SMTseq4	Input	19,111	19,287	19,630	19,343	23,573,272	24,615,731	30,217,161	26,135,388
SMTseq4	IP	19,835	20,551	20,488	20,291	14,407,184	19,577,709	17,262,557	17,082,483
SMTseq0.25	Input	16,834	16,742	16,117	16,564	23,815,261	24,347,672	22,951,893	23,704,942
SMTseq0.25	IP	16,827	16,870	16,527	16,741	13,971,566	15,047,219	14,691,099	14,569,961

ISSN: 1471-2164