Fig. 13

This figure depicts the qRT-PCR analysis of seven “lipogenic” DEGs identified by genome array analysis. Each value represents the mean ± SEM of four biological samples (individual embryo or hatchling liver) and their two technical replicates in the qRT-PCR analysis. Values possessing different superscripts are significantly different as determined by ANOVA and mean separation using Duncan’s Multiple Range Test in SAS. These genes are mainly involved in lipolysis and energy catabolism; and all are expressed at higher levels in embryos, with the exception of the invariant gene cytochrome c oxidase subunit 7A2 like (COX7A2L). The invariant gene was one of three housekeeping genes used for normalization of transcript abundance in liver of embryos and hatchling chicks. Gene symbols: Monoacylglycerol O-acyltransferase 1(MOGAT1); pyruvate dehydrogenase kinase 4 (PDK4); frizzled class receptor 2 (FZD2); lactate dehydrogenase B (LDHB); iodothyronine deiodinase 3 (DIO3); iodothyronine deiodinase 1 (DIO); and adiponectin, C1Q and collagen domain containing (ADIPOQ)