Fig. 7

This gene network (Panel a) from the E18 vs. E20 contrast was functionally annotated by IPA as “Lipid Metabolism”. It shows interactions among four nuclear hormone receptors [nuclear receptor subfamily 0 group B member 2 (NROB2), estrogen related receptor gamma (ESSRG), peroxisome proliferator-activated receptor-alpha (PPARA) and PPARGC1A] that regulate energy balance and lipid metabolism in liver of late embryos. In Panel b, Ingenuity® Upstream regulator Analysis predicts that PPARGC1A itself is inhibited (blue symbol) because 16 known target genes are down-regulated in the E18/E20 contrast. The blue arrows predict that 11 known target genes should be inhibited. Actually, red-colored gene symbols indicate higher expression in liver of E18 embryos, whereas the green-colored symbols show higher expression in E20 embryos. In addition, Ingenuity predicts that activated NROB2 leads to inhibition (blunt blue lines) of seven lipogenic genes (PPARGC1A, ANOX1, CPT1A, CTP8B1, EGR1, ESRRG and HMGCR), whereas, the upregulation of NR1H4 is inconsistent with its expected state (based on the Ingenuity Knowledge Base)