Table 6 IPA summary of the liver transcriptome in the E20 vs. D3 contrast

Top Canonical Pathways

p-value

Overlap

Ratio

 Super-pathway of Cholesterol Biosynthesis

1.97E-09

69.6%

16/23

 Cholesterol Biosynthesis I-III

1.82E-07

90.0%

9/10

 Complement System

5.41E-07

58.3%

14/24

 LXR/RXR Activation

7.87E-06

34.7%

25/72

 Intrinsic Prothrombin Activation Pathway

2.00E-05

55%

12/20

Top Upstream Regulators

p-value of overlap

# Target genes

 TP53

2.52E-23

257

 PPARA

1.23E-20

100

 E2F4

3.03E-20

71

 E2F1

5.46E-18

105

 FOXO3

6.20E-16

77

Top Molecular and Cellular Functions

p-value

# Genes

 Cell Cycle

8.31E-04 - 5.90E-14

392

 Cellular Assembly and Organization

8.35E-04 - 5.90E-14

188

 DNA Replication, Recombination, and Repair

8.31E-04 - 5.90E-14

294

 Lipid Metabolism

8.39E-04 - 1.88E-11

333

 Small Molecule Biochemistry

8.39E-04 - 1.88E-11

382

Physiological System Development and Function

p-value

# Genes

 Organismal Survival

2.88E-04 - 1.37E-05

470

 Connective Tissue Development and Function

2.59E-04 - 1.65E-05

152

 Tissue Morphology

2.88E-04 - 1.65E-05

219

 Reproductive System Development and Function

8.08E-04 - 4.69E-05

10

 Embryonic Development

5.07E-04 - 7.98E-05

35

Top Toxicity Functions

p-value

Overlap

Ratio

 LXR/RXR Activation

1.04E-05

34.2%

25/73

 Cholesterol Biosynthesis

4.67E-05

60.0%

9/15

 Fatty Acid Metabolism

5.58E-05

33.3%

22/66

 Cell Cycle: G2/M DNA Damage Checkpoint Regulation

6.76E-05

39.0%

16/41

 FXR/RXR Activation

1.38E-04

30.4%

24/79

Top Up-regulated Genes

log2 Ratio

Top Down-regulated Genes

log2 Ratio

 MOGAT1

5.27

THRSPA

−9.13

 PDK4

5.04

CDO1

−6.73

 ITGBL1

4.50

SQLE

−6.70

 CPT1A

4.27

NSDHL

−6.62

 PPM1K

4.16

FASN

−5.84

 DNAJC12

3.91

SCD

−5.80

 IGFBP2

3.68

ELOVL5

−5.51

 CPED1

3.66

FADS2

−5.46

 ZBTB16

3.37

CDKN2B

−5.01

 MT3

3.36

CYP7A1

−4.98