Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices

BMC Genomics

Table 2 Oxford nanopore technologies MinION sequencing yields and basic details and breakdown of centrifuge classification

	Total		Mean	Median	Low complexity		Human		Bacteria
Sample	Bases	Reads	read length	read length	bases	reads	bases	reads	bases	reads
229a	204,346,556	124,218	1645.06	1745	113,836	69	198,972,861	117,821	1,692,097	914
249a	723,925,562	585,098	1237.27	1006	403,668	370	563,888,189	411,612	44,502,912	34,773
259a	1,057,865,247	600,291	1762.25	1321	390,209	289	949,663,786	502,426	512,827	312
312a	1,121,119,742	1,004,818	1115.74	674	1,905,423	1044	1,038,235,876	882,763	30,426,198	14,948
335a	2,847,687,425	1,717,810	1657.74	1171	2,835,054	1362	2,783,128,118	1,605,466	1,388,748	989
352a	803,638,340	986,867	814.33	609	567,656	630	669,796,136	752,022	459,779	579
354a	706,380,170	945,929	746.76	596	680,560	848	570,485,740	717,662	2,151,551	2443
509a	2,740,060,527	4,940,241	554.64	439	16,355,839	24,413	1,199,779,866	1,352,438	6,240,425	2628
506a	2,451,399,949	4,700,013	521.57	431	20,014,343	23,631	1,161,796,584	1,671,726	4,705,919	2139

Bacteria, Human with a centrifuge score greater than 150, and total reads including unclassified reads. Samples 509a and 506a are culture negatives and used as negative controls. Results are after removing low complexity reads

ISSN: 1471-2164