Fig. 1
Karyotypes from three PFGE runs. 1% Megabase agarose gels (Bio-Rad) were loaded with agarose plugs bearing lysates of ~ 1 × 107 epimastigotes of each trypanosomatid strain for electrophoresis at 13.5 °C using the CHEF DR III System (Bio-Rad). Run conditions used for karyotyping each species were based empirically on their individual distributions of chromosome sizes. For separation of smaller chromosome size ranges, we used the following program - Block 1: 5 V/cm, 20–200 s, 18 h, 120°. Block 2: 3 V/cm, 200–300 s, 32 h, 120°. Block 3: 1.5 V/cm 500–1100 s, 12 h, 120°. The program used for separation of the largest chromosome size ranges was as follows - Block 1: 2 V/cm, 1500 s, 12 h, 98°. Block 2: 2 V/cm, 1800 s, 12 h, 106°. Block 3: 3 V/cm, 500 s, 38 h, 106°. Block 4: 5 V/cm, 20–200 s, 23 h, 120°. Block 5: 3 V/cm, 200–400 s, 34 h, 120°. (a) T. rangeli AM80 vs. T. conorhini 025E using Saccharomyces cerevisiae chromosome size-markers (Bio-Rad). (b) T. conorhini 30028 vs. T. conorhini 025E. (c) T. cruzi G vs. T. cruzi CL. Schizosaccharomyces pombe, Hansenula wingei and Saccharomyces cerevisiae chromosomes (Bio-Rad) were used as markers for (b) and (c)