miRNA | log2FC | Target gene | log2FC | Signaling pathway | Function |
---|---|---|---|---|---|
miR-1343 | 1.6303 | PDE4A | – | cAMP-PKA | Regulating the concentration of local cAMP and sperm capacitation [45] |
miR-1285 | −2.9161 | AKAP3 | −6.5979 | MAPK | Regulating the level of AKAP3 tyrosine phosphorylation [71] |
miR-127 | −3.8252 | VDAC1 | −5.5646 | Calcium | Affected the function of sperm motility, survival rate, acrosome reaction, capacitation, tyrosine phosphorylation, fertilization and embryo development [46, 47] |
miR-151-3p | 1.7770 | CATSPER4 | −5.7323 | Calcium | Affect the concentration of intracellular calcium and forward movement of sperm [72] |
miR-133a-3p | −26.7016 | PRDX5 | −8.2880 | – | Preventing oxidative stress during human sperm capacitation [73] |
miR-378 | −2.3458 | DNM1 | −5.0628 | – | Regulation of human sperm acrosomal exocytosis [74] |
miR-1306-3p | 1.5420 | CLU | −7.2109 | – | Important for sperm maturation and capacitation [75] |
miR-214 | −25.9240 | CYP19A1 | −5.4823 | – | Increasing the translational activities during capacitation for more protein synthesis [76] |
conservative_3_158937 | 1.0748 | MYC | −6.9168 | ||
conservative_7_221178 | 3.5235 | PSPI | −5.7360 | – | Participating in the regulation of reproductive immunity, and the process of fertilization; maintaining sperm viability, exercise ability, and mitochondrial activity [77] |
unconservative_7_234335 | 1.7749 | CABYR | −9.1633 | – | Calcium binding tyrosine phosphoric acid fiber vitamin sheathing protein, participates in sperm capacitation [28] |
unconservative_11_42222 | 1.8879 | ACRBP | −4.8677 | – | Promotion of acrosin maturation and sperm capacitation [53] |