Fig. 7

Co-expression patterns of late cup and prestalk or prespore marker genes. Cells were co-transformed with fusion constructs of RFP and the cotC or ecmB promoters, and of YFP with the beiB promoter. Co-transformed cells were developed to fruiting bodies on a thin layer of agar. A small portion of the agar was excised for imaging of spore heads using a Leica TCS SP8 confocal microscope detecting RFP or YFP fluorescence. a, b Spore heads co-expressing [cotC]:RFP and [beiB]:YFP. Left and centre panels show RFP and YFP fluorescence, respectively, and in the right panel RFP and YFP fluorescence are superimposed. a intact spore head, b squashed spore head at higher magnification showing the absence of RFP and YFP co-localization, except for one cell (marked by dashed circle). c Spore head showing co-expression [ecmB]:RFP and [beiB]:YFP. The first panel is a phase contrast image of the spore head. The remaining panels show [ecmB]:RFP, [beiB]:YFP and superimposed fluorescence images as indicated. Note that there is almost complete overlap between ecmB and beiB expression. Scale bar = 50 μm