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Table 3 Comparison of quality control of average sequencing data for four kits

From: Metagenomic analysis of viral nucleic acid extraction methods in respiratory clinical samples

Kit Raw dataa(G) Clean datab(G) Remove hostc(G) Clean Q30d(%) Effective Ratee(%)
VRMK 2.43 2.34 0.66 93.33 26.73
MVSK 2.77 2.73 0.32 95.18 12.10
RPMK 2.91 2.85 1.88 93.60 67.47
RMK 2.42 2.34 0.63 93.26 26.79
  1. aReads were not processed with bioinformatics analysis
  2. bReads were quality-filtered by removing low quality bases, reads shorter than 75 bp, and reads with low entropy
  3. cHuman reads were removed by aligning human (hg19) reference genome
  4. dhe percentage of bases with a quality score greater than 30
  5. eRemove host /clean data
  6. The best result is presented in bold