Fig. 4

Core CNC networks during the CN-induced osteoblast differentiation of MSCs. a Core CNC network based on the 29 core genes obtained from GO cluster analysis and KEGG pathway analysis. b Sub-core CNC networks based on the 6 core genes obtained from online databases and core CNC networks. c qRT-PCR verification of miRNA expression. Compared with the control group, the expression levels of all the core miRNAs were lower in the CN-induced MSC group, which was consistent with the sub-core CNC network results. d qRT-PCR verification of lncRNA expression. Compared with the control group, the expression levels of all the core lncRNAs were higher in the CN-induced MSC group, which was consistent with the sub-core CNC network results. e qRT-PCR verification of mRNA expression. Compared with the control group, the expression levels of all the core mRNAs were higher in the CN-induced MSC group, which was consistent with the sub-core CNC network results. f and g Western blot verification of mRNA expression; the results were similar to the qRT-PCR results. P < 0.05, compared to the control group