Fig. 6

miR-298 negatively regulated osteogenesis-related factors during CN-induced MSC osteoblast differentiation. a AR-S showed that more calcium mineral deposition was found in the miR-298 inhibitor group than in the miR control and miR-298 mimic groups; in other words, miR-298 suppressed MSC osteogenic differentiation. b qRT-PCR detection of the mRNA expression of osteogenic-related factors in MSCs transfected with miR-298 mimic or miR-298 inhibitor showing that the mRNA levels of the osteogenic-related factors were decreased in the miR-298 mimic group compared with those in the miR control group, whereas these levels were enhanced in the miR-298 inhibitor group. c and d Western blot detection of the protein expression of osteogenic-related factors in MSCs transfected with miR-298 mimic or miR-298 inhibitor; the results were similar to the qRT-PCR results. miR control group: MSCs transfected with miR control; miR-298 mimic group: MSCs transfected with miR-298 mimic; miR-298 inhibitor group: MSCs transfected with miR-298 inhibitor. *P < 0.05 vs. the miR control group. #P < 0.05 vs. the miR-298 mimic group