Correcting palindromes in long reads after whole-genome amplification

BMC Genomics

Table 1 Effect of correcting palindromes

Sample	Before cleaning		Reads with detectable palindromes		After correcting
	Number of reads	Average length (b)	Number of reads	Number of reads (%)	Number of reads	Average length (b)
Ath-WGA1	462,138	9326	221,001	47.8	869,826	4660
Ath-WGA2	447,364	8544	195,263	43.6	769,027	4721
Ath-Ctrl	940,162	5680	4714	0.5	938,196	5681
GorY-WGA	3,596,236	5468	426,188	11.8	4,546,488	4234

Effect of correcting palindromes on the number reads and average lengths of these reads. Note: the Ath-Ctrl shows a small increase in average read length after correction and a lower number of reads. This is because Pacasus removes very short reads from the output

ISSN: 1471-2164