Fig. 4

Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, (b) Clone H8 (ybeM), (c) Clone A2 (ybeM), (d) Clone H9 (ybeM), (e) Clone C2 (ybeM), (f) Clone D10 (ybeM), (g) Clone C1 (ybiY), (h) Clone A4 (ybiY), (i) Clone A11 (ybiY-moeA). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in (j) and (k) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]