Skip to main content

Table 3 Target genes and primer sequences for real time-PCR analysis of gene expression in mouse adipose tissue

From: Differential effects of coconut versus soy oil on gut microbiota composition and predicted metabolic function in adult mice

Gene name

Entrez Gene Id

Forward primer

Reverse primer

Product length

Adiponectin (Adipoq)

11,450

TGTTCCTCTTAATCCTGCCCA

CCAACCTGCACAAGTTCCCTT

104 bp

Leptin (Lep)

16,846

GAGACCCCTGTGTCGGTTC

CTGCGTGTGTGAAATGTCATTG

139 bp

Tumor necrosis factor alpha (TNF-α)

21,926

CCCTCACACTCAGATCATCTTCT

GCTACGACGTGGGCTACAG

61 bp

Ribosomal protein L32 (Rpl32)

19,951

TTAAGCGAAACTGGCGGAAAC

TTGTTGCTCCCATAACCGATG

100 bp

  1. Primers sequences were taken from PrimerBank database