Fig. 2

Detection of genes potentially regulated by radiation-activated p53 and NF-κB by integrative genomics. a Flowchart of the study. b Number of genes upregulated and downregulated by irradiation (at either 4 or 10 Gy dose) in wt cells, where IR induced or enhanced binding of either RelA or p53 in regulatory regions (listed are protein-coding genes with the binding of both transcription factors). c Number of genes with expression significantly affected by radiation (upregulated/downregulated, respectively; at either dose), whose expression was either inhibited or stimulated in cells with siRNA-silenced RELA or TP53 (listed are protein-coding genes whose IR-modulated expression was affected by silencing of both transcription factors). d Number of radiation-upregulated genes with IR-stimulated promoter binding of either RelA or p53 (in wt cells), which IR-modulated expression was affected in cells with siRNA-silenced RELA or TP53 (listed are “TF-dependent” genes, i.e., protein-coding genes with expression affected by silencing of transcription factor (TF) where radiation stimulated promoter binding of the corresponding TF)