Fig. 4

Genes putatively co-regulated by NF-κB and p53; validation of genomics profiling. a The influence of silencing of RELA and TP53 on gene expression analyzed by RT-qPCR in the same set of genes 4 h after irradiation with 10 Gy. Expression levels are presented as a fold-change vs. untreated wild-type control cells (dots represent mean values); asterisks indicate statistical significance of differences between cells treated with control siRNA and cells treated with siRNA specific for RELA or TP53: * p < 0.05, ** p < 0.001. b Radiation-stimulated binding of RelA (p65) and p53 in putative regulatory regions of selected genes analyzed by ChIP-qPCR; p53 binding was additionally illustrated by gel electrophoresis of PCR products (C – control, 2 and 4–2 and 4 h after irradiation with 10 Gy). Marked are median, maximum and minimum values of a fold-change vs. untreated control (asterisks indicate statistical significance of differences: *p < 0.05, **p < 0.001)