Fig. 3

Overview of peach transcriptome responses to aphid feeding. a Principal component analysis (PCA) analysis of 3854 genes identified by transcript profiling (RNA-seq) of R36 and S38 infested with aphids for the indicated time periods. Hours of aphid infestation are indicated by the number following the R (R36) or S (S38) cultivar, i.e., R0h indicates 0 h of aphid infestation in R36. b Hierarchical clustering of all differentially expressed genes. Distances were calculated using Pearson’s similarity, and agglomeration was performed according to Ward’s minimum variance algorithm. The heat-map diagram shows the relative expression level at the eight time points (0, 3, 6, 9, 12, 24, 48 and 72 h after aphid infection) for two genotypes