Fig. 2

Application of PorSignDB to lymph node data originating from pig farms with naturally occurring PMWS. a Outline of the analysis. Data from PMWS-affected farms were retrieved from GEO. In PMWS lymph nodes, follicular structures become indistinct and B-cells and T-cells all but disappear, while infiltrating macrophages fuse into multi-nucleated giant cells. In PCV2-positive healthy lymph nodes, lymphoid structure is intact. Comparing transcriptomes of both phenotypes using GSEA displays enrichment of PorSignDB transcriptional signatures. Clip art was made by NVR. b Microbiology-related PorSignDB gene set expression in lymph nodes of PMWS pigs versus Healthy pigs (FDR < 0.01 and opposite expression of each pairwise phenotype). The average expression of the leading-edge genes in every gene set (genes that contribute to the enrichment) are displayed for each patient sample. Bars next to each gene set indicate the signed FDR for its enrichment in log10 scale. c Temporal performance of PorSignDB’s Subclinical PCV2 29 dpi infection signatures in subclinically infected pigs