Fig. 8

Pathway analysis (a), as well as genomic locations (b) and gene expression (C) of lncRNA-PCG pairs that were differentially regulated by lack of gut microbiota in Ileum of CV and GF mice. (a) The PCGs paired with lncRNAs in ileum between CV and GF mice were subjected to STRING analysis using the default settings. The connected nodes are shown. (b) and (c) The first rate-limiting enzyme of glutathione synthesis glutamate-cysteine ligase catalytic subunit (Gclc), the rate-limiting enzyme for cholesterol synthesis 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), and the cholesterol synthesis enzyme 3-hydroxy-3-methylglutaryl-CoA synthase 1 (Hmgcs1) are shown. Expression of lncRNAs and paired PCGs were plotted using mean FPKM ± S.E., and asterisks (*) indicate statistically significant differences between enterotypes of mice (FDR-BH < 0.05)