Fig. 3
From: ddSeeker: a tool for processing Bio-Rad ddSEQ single cell RNA-seq data
Application of ddSeeker to in-house dataset a Dot plot of the percentage of valid reads (PASS) and reads with errors in barcode and/or UMI in the low (Run 1) and high (Run 2) sequencing quality read sets. Details regarding error classification are reported in Additional file 1. b Number of reads per cell barcodes across the 5000 most read barcodes in the two read sets. Vertical dashed line indicates the number of expected cells (1800) in our libraries based on ddSEQ specifics. c Cumulative fraction of reads per cell in the 5000 most read barcodes in the two read sets. All these plots can be generated with make_graphs.R, a dedicated R script included in the ddSeeker package