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Fig. 3 | BMC Genomics

Fig. 3

From: Homologous recombination changes the context of Cytochrome b transcription in the mitochondrial genome of Silene vulgaris KRA

Fig. 3

Structure of the autonomous chromosome 5 of S. vulgaris KRA. Southern blot hybridization probed with 626 bp long sequence specific to the chromosome 5. Total DNAs were extracted from leaf (L) or flower bud (B) tissues of female (F) and hermaphroditic (H) plants. DNAs were digested with the restriction enzyme BglII recognizing a single site in chromosome 5 (right), treated with nickase Nb.BsrD1 capable of introducing one single-strand cut in chromosome 5 (middle), or not digested at all (left). The arrows point at the bands corresponding to open (relaxed) circle, linear (1.56), or supercoiled monomer of chromosome 5

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