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Table 8 Target genes identified by 5’ RACE sequencing

From: Identification and characterisation of microRNAs and their target genes in phosphate-starved Nicotiana benthamiana by small RNA deep sequencing and 5’RACE analysis

miRNA IDa

Nearest homologue

Target

Clones matching

Region of cleavage site

B201_2_802950_21_4_mir166

Sly-miR166

Homeobox-leucine zipper protein ATHB-15 (probable)b

12/12

Coding

benth-B97_3_848_21_3_mir319

Sly-miR319

Transcription factor GAMYB (probable)b

15/15

Coding

B1569_1_1026_21_2_mir398

Nta-miR398

Superoxide dismutase

[Cu-Zn] (similar to)b

4/17

5’UTR

Blue copper binding protein (probable)b

11/14

5’UTR

B465_1_1169_21_2_mir399

Ath-miR399b

Probable ubiquitin-conjugating enzyme E2 24 (probable)b

14/14 (Site two)

12/12 (Site four)

5’ UTR

B29_6_545_21_6_mir399

Ptc-miR399d

Inorganic phosphate transporter 1–4 (probable)

11/11

Coding

B947_1_816_21_1_NA

Ptc-miR399f/g

B246_2_947_21_2_NA

Nta-miR6147

Putative late blight resistance protein homolog R1B-16 (probable)

12/17 (Site two of two)c

Predicted as Coding

benth-B326_1_154_20_1_NA

benth-B1461_1_95_20_1_NA

Ath-miR2111a-5p

F-box/kelch-repeat protein At3g27150 (probable)b

8/26

Coding

  1. amiRNA ID from Nicotiana benthamiana small RNA sequencing reads
  2. bTarget previously confirmed in other species
  3. cCleavage point in all clones at the first nucleotide of miRNA binding site