Fig. 1

High-throughput ChIPmentation (HT-CM) through direct amplification of tagmented chromatin, allows for rapid and technically simple analysis of histone modifications and transcription factor binding in low numbers of FACS sorted cells. a Schematic overview of the HT-CM workflow (for a direct comparison between the HT-CM and original ChIPmentation (CM) methods, see Additional file 1: Figure S1). In brief, FACS sorted cells are sonicated, subjected to ChIP and tagmented. Library amplification is subsequently done without prior DNA purification. Input controls are prepared through direct tagmentation of sonicated chromatin. b Genome-browser profiles from CM, HT-CM and input control samples generated using indicated cell-numbers and antibodies. c Correlation between H3K27Ac signals (in a merged catalog containing all peaks identified in displayed samples) generated using indicated methods and cell numbers. d Overlap (%) between top peaks (peaks with the 50% highest peak quality scores) identified in high cell-number (150 and 50 k) H3K27Ac HT-CM and CM samples. e RPKM of 1 kb bins covering the whole genome in input control samples generated using indicated method and cell-equivalents of chromatin. f Percentage of unique reads in H3K27Ac HT-CM and CM samples generated in parallel. g Correlation between H3K27Ac/CTCF signals in samples generated using indicated methods and cell-numbers. h Overlap (%) between top peaks identified in H3K27Ac and CTCF HT-CM samples generated using indicated cell-numbers. ND, not done. i Time required to perform ChIP, library preparation and sequencing for the CM, HT-CM and 1-day HT-CM workflows. Hours (h) needed to perform each step are indicated