Fig. 3

Multiple alignment of conserved domains of proto-oncogenes proteins and cell type-specific expression patterns of these proto-oncogenes. a Alignment of S_TKc domain of P. brassicae Raf proto-oncogenes with selected homologs. (GenBank accession nos.: Blastocystis sp., OAO16337.1; Malus domestica, XP_008390530.1; Prunus mume, XP_008223365.1; Camelina sativa, XP_019092831.1; Malus domestica, XP_008390530.1; Nicotiana sylvestris, XP_009791599.1; Solanum tuberosum, XP_015169630.1; Acanthamoeba castellanii, XP_004334709.1; Polysphondylium pallidum, EFA76341.1; Homo sapiens B-raf proto-oncogene protein, AAA96495.1; Homo sapiens RAF1 protein, AAA60247.1). b Alignment of myb domain of P. brassicae proto-oncogene protein Pbmyb with its homologs. (GenBank accession nos.: Rhagoletis zephyria, XP_017478845.1; Bactrocera latifrons, XP_018796786.1; Alligator mississippiensis, XP_019340180.1; Camelina sativa, XP_019092831.1; Homo sapiens MYB proto-oncogene protein, AAA52031.1; Dictyostelium fasciculatum, XP_004355527.1; Physcomitrella patens, AAF78887.1). Identical residues are shaded in black and gray, and gaps are indicated by dashed lines. Alignments were generated by using the ClustalW algorithm with additional manual adjustments. c The cell type-specific expression patterns of proto-oncogenes from P. brassicae were measured by RNA-seq data (red line chart) and qRT-PCR data (black histogram). The P. brassicae actin gene was used as an internal control to normalize the expression data. The histograms and error bars represent means and standard deviations of qRT-PCR, respectively. Pearson’s correlation coefficient (R-value) was used to measure the consistency of the RNA-seq data and qRT-PCR. See Additional file 3: Table S1 and Additional file 6: Table S4 for detail of genes