Microsatellite development from genome skimming and transcriptome sequencing: comparison of strategies and lessons from frog species

Table 1 Overview of the sequencing data from Illumina next-generation sequencing for each species of frog

NGS Source	Species	Total sequence reads (Million)	Total No. of bases (Gigabase)
Genomic^a	Amolops chunganensis	7.01	4.21
	Amolops mantzorum	10.19	6.11
	Hyla chinensis	6.93	4.16
	Hylarana latouchii	6.63	3.98
	Quasipaa boulengeri	8.28	4.97
	Rhacophorus chenfui	7.28	4.37
Transcriptomic^b	Amolops mantzorum	52.28	5.04
Transcriptomic^b	Quasipaa boulengeri	73.25	7.32

^a Genomic samples were sequenced using the Illumina Miseq (PE300) system
^b Transcriptomic samples were sequenced using the Illumina Hiseq 2000 (PE125) system

ISSN: 1471-2164